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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and thus may well lead to tumorigenesis. This protein was identified to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.
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Blocking peptides are peptides that bind exclusively on the goal antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged with the antibody.
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This guidebook outlines additional probable optimization of cellular and immunoassay parameters to be sure the very best final results are...
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This guideline outlines more probable optimization of mobile and immunoassay parameters to guarantee the absolute best results are...
Antibodies bound check here to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining in the blocked antibody compared to the antibody alone, one can see which staining is distinct.
Goal Details Cyclin E belongs into the very conserved cyclin loved ones, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation designs which lead into the temporal coordination of each mitotic party. Cyclin E kinds a complex with and features as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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